https://www.roepmanlab.com/home daily 1.0 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1585294034454-PY3X7E68NAHZ1KWQ5MPJ/DSC_7291.jpeg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1586943360814-1LY0DZSQ5ZWCR5FSPTMT/cilia_3.jpeg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1586943388957-20NG5CLFD9OPKYUT98Q3/cilia_1.jpeg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1586941963967-ZVZXRY01JT5078TOAZMP/closeup%2Bcilium.jpg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1586943450276-MSJ5OKND8JTHZ055U11Q/closeup+cilium_2.jpg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1588872552597-OT9C6YQXME63OW1KRNC6/Rosette_DAPI_Nestin_GFAP_1.jpg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1588872569510-5DTC65ZVT073FDVZQBYK/Rosette_MAP2_PAX6_1.jpg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1585293939151-KAPQTHEK30SJC960JEC5/DSC_7292.jpeg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/dd186e15-a4cd-44aa-ada2-cf6c3719a7cc/IMG_6516.jpg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/2cb9ebcb-92fa-453b-8cb6-24a32a7afc7e/Kae+retinal+organoid.png Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/2abbd380-0a47-4196-8fc3-c255da9e28fa/IMG_20231005_180908.jpg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/167de3ec-8cb8-4ad8-9a99-f54146e86979/LCA5+UxEM.png Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/0f26be25-7c7b-4fda-bd33-72adaf41369c/IMG_20231006_144752.jpg Home https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/7e953844-7439-499b-8744-48e348458b42/IMG_6354+2+Small.jpeg Home https://www.roepmanlab.com/vacancies daily 0.75 2025-01-15 https://www.roepmanlab.com/ronald-roepman daily 0.75 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1589734626699-5CO2HTKKOKCHPSU2D428/RonaldRoepman_2016.jpg Ronald Roepman https://www.roepmanlab.com/sylvia-van-beersum daily 0.75 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1585924925612-KQI0XIQ9TLT17MW9GATB/syl.jpg Sylvia van Beersum https://www.roepmanlab.com/ideke-lamers daily 0.75 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1585925202541-215NCMVN2J82UWBY2IMA/ideke_lab.jpg Ideke Lamers https://www.roepmanlab.com/mariam-aslanyan daily 0.75 2024-01-03 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/8e62ebfc-9bd5-4dee-be58-ba4ba2da5689/IMG_5461+copy.jpg Mariam Aslanyan https://www.roepmanlab.com/stef-letteboer daily 0.75 2024-01-15 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1586436857197-PP7KMHLLMO5AUSENLK7A/Z514125.jpg Stef Letteboer https://www.roepmanlab.com/nikoleta-argyrou daily 0.75 2024-01-04 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1586437182590-AO7MO9K74REJMFJOUWIW/photo_Nikoleta.jpg Nikoleta Argyrou https://www.roepmanlab.com/simone-dusseljee daily 0.75 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/bd6e16d2-a095-4ff1-987c-715cff354984/Simone+foto.JPG Simone Dusseljee https://www.roepmanlab.com/former-lab-members daily 0.75 2024-01-02 https://www.roepmanlab.com/students daily 0.75 2021-03-26 https://www.roepmanlab.com/tandem-affinity-purification daily 0.75 2023-01-25 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1586956351728-5RFHDLMEIPG1GTH1R44Z/Overview-of-the-SF-TAP-procedure-a-In-the-first-purification-step-I-the-tandem.jpg Tandem Affinity Purification Gloeckner et al, Methods Mol Biol, 2009 https://www.roepmanlab.com/y2h daily 0.75 2023-01-25 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1588357443310-27DRJWSLM6VI9K6MNN7Y/Screenshot+2020-05-01+at+20.21.26.png Yeast Two-Hybrid Fig. 2. Assessment of the results from yeast two-hybrid screening. (A) Detection of activation of the MEL1 reporter gene by a colorimetric X-α-gal plate assay, detecting α-galactosidase production and secretion by the growing yeast colonies. (B) Detection of activation of the LacZ reporter gene by a colorimetric X-β-gal plate assay, detecting β-galactosidase production in the yeast cytoplasm by a filter-lift assay. https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1588357334492-2QGVV6F55TXPQRGR1W56/Screenshot+2020-05-01+at+20.20.37.png Yeast Two-Hybrid Fig. 1. Yeast two-hybrid mating strategy. A plasmid expressing a library prey protein (Y) fused to the GAL4 transcrition activation domain (AD) is transformed into a yeast cell of mating type α. Upon combination (mating) with a yeast cell of the opposite mating type (A) expressing the bait protein (X) fused to the GAL4 DNA binding domain (BD), a diploid yeast cell is formed, allowing the protein-protein interaction and consequently transcription of the reporter genes to take place. This is assessed by detecting growth on media lacking histidine and adenine, the development of a blue/green color by a plate assay detecting α-galactosidase, and detection of β-galactosidase by a LacZ filter-lift assay. https://www.roepmanlab.com/bioid-proximity-labeling daily 0.75 2020-04-15 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1586971430374-QGIEV3YCMGPFT4BA3TRT/BioID-The-protein-of-interest-is-fused-with-a-promiscuous-form-BirA-of-the-bacterial.png BioID proximity labeling Renata Varnaite and Stuart A. MacNeil, Proteomics, 2016 https://www.roepmanlab.com/crispr daily 0.75 2023-01-25 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1589736384473-TLLC6660H8M7FOACQGLZ/Screenshot+2020-05-17+at+19.25.25.png Crispr Plumer et al., Vox, 2018 https://www.roepmanlab.com/news daily 0.75 2024-03-25 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/786f8905-0685-4a74-8001-f3ea6fb29782/Kickoff+Meeting+CilNetNL+group+picture.jpg https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/ef3c44b0-8870-4556-9af6-2e87fa50f60d/3bf2d656-ba9b-4dcb-8243-b4cfb1d2012c.JPG https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/e07270b4-74bd-49d3-a815-2605f12ed8cc/Screenshot+2024-01-02+at+19-07-52+%282%29+WhatsApp.png https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/251f6589-ca7f-426d-98d7-150fb7528953/IMG_6523.jpg https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1588876885076-5496ZANSYH50X4F32DHK/ProfilePhoto_Cenna.jpg https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/bd61f5ad-ee79-43da-abad-424b7d15d3cf/IMG_6524.jpg https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/c5be3053-e8f1-4ee9-958c-c454a2a67274/IMG_6522.jpg https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/d51a4d71-c497-44b8-8014-ace89ca8f4bc/Screenshot+2024-01-03+at+09-47-20+brooke+latour+thesis+radboud+-+Google+Search.png https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/4903e113-a3d3-48a0-b0f1-83ccd6dd118d/IMG_6521.jpg https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1616787491340-A737N2YE4VNH09VTZS9Y/Photo+26-03-2021%2C+20+30+15.jpg News https://www.roepmanlab.com/education daily 0.75 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1587676721554-TVRXPMFXAQZ84VH0BOE0/InScienceFilmFestival_dec2019_DesignerBabiesStand_Ideke01.jpg Education https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1587677172089-T6NO4CT2TJP91G30A8DM/InScienceFilmFestival2019_Day3_Fotograaf_Almicheal_Fraay-40.jpg https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1587672617920-3UG6V5NEF9VEUGP3EP18/Campagnebeeld+InScience+2019.png Education https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1587672619366-SRBPKQEUGYTZ0ZPHI8ZK/InScienceFilmFestival_dec2019_DesignerBabiesStand_Ideke02.jpg Education https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1587672680509-L2Q7XNQW7NWZ66PSWBV4/InScienceFilmFestival2019_Day3_Fotograaf_Almicheal_Fraay-39.jpg Education https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1587672669835-JAY8WZVARL39N0GEQJKK/Maros+Pulscak2.jpg Education https://www.roepmanlab.com/ciliary-signalling daily 0.75 2023-01-25 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1588188112330-7JARW0711R3R2WP437JH/Screenshot+2020-04-29+at+21.20.57.png Ciliary signalling https://www.roepmanlab.com/kae-whiting daily 0.75 2024-01-08 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/9ad59380-1f93-49e5-a456-72d7a531a1df/Lab+Page+Photo.jpg Kae Whiting https://www.roepmanlab.com/new-page-21 daily 0.75 2024-01-03 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/b10d1412-04db-4753-adfc-c00bcaa83397/cilia+strucutre.001.jpeg WHAT ARE CILIA - Make it stand out Figure 1. Schematic structure of the cilium. Cilia are composed of a basal body (BB) and a ciliary axoneme that protrudes from the apical plasma membrane. These two structures are linked via the transition zone (TZ) that consists of three compartments, i.e. a base, and two compartments filling the space between the microtubules and the axonemal membrane. Structurally the TZ is defined by the presence of Y-shaped linkers, which together form the ‘ciliary necklace’ that is wrapped around the base of the cilium, as shown in the electron microscopy image at the bottom right corner. The TZ acts as a diffusion barrier and regulates ciliogenesis and signaling. Cilia contain a microtubule cytoskeleton that is composed of nine microtubule doublets organized in a ring, with the plus-ends situated at the ciliary tip. A central microtubule pair is present only in motile cilia. These microtubules together with inner and outer dynein arms, radial spokes, and nexin links drive ciliary movement. To generate movement dynein heavy chains of one doublet slide against microtubules of a neighboring doublet thereby orchestrating the beating of the cilium in an ATP-dependent fashion. In both cilium types the ciliary microtubules https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/a3ab04b3-ad62-41c2-ac1c-ec7d351fcd9b/SHH+for+website.001.jpeg WHAT ARE CILIA - Make it stand out Figure 2. Shh pathway overview. When no Shh ligand is present, the Patched-1 (PTCH1) receptor represses Smoothened (SMO) from activating Gli2 and Gli3 transcription factors and entry of SMO into the ciliary membrane. Furthermore, the Gli transcription factors are bound by SUFU. When Shh ligand is present, PTCH1 is activated and will release SMO, allowing its ciliary entry and translocation to the tip. This will activate and release the Gli transcription factors from SUFU, allowing them to translocate from the cilium to the nucleus and activate downstream target genes important for neuronal and skeletal development. These Gli transcription factors are bi-functional and can also actively repress transcription. When the pathway is not activated, Gli2 and Gli3 are phosphorylated by PKA, CKI and GSK3β, causing proteolytic cleavage which generates repressor forms (Gli2R and Gli3R). PKA is thought to be activated by increased cAMP levels generated by GPR161. Activation of the Shh pathway promotes the exit of GPR161 from the cilium, and thereby stops Gli2/3 phosphorylation and generates high concentrations of activating (uncleaved) transcription factors. The precise balance of activator and repressor forms of the Gli transcription factors determines the gene expression pattern of the cell and thereby its differentiation into a specific progenitor subtype. https://www.roepmanlab.com/ciliary-microtubule-dysregulation daily 0.75 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1588184561945-OOK8O4TIPG7FFMVCNBXJ/zonmw-logo-og-1.jpg https://www.roepmanlab.com/mproving-diagnosis-of-ciliopathies daily 0.75 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/cd3cbd24-df0f-46e1-861a-964ab7e8375e/Screenshot+2024-01-02+at+15-44-41+European+Joint+Project+on+Rare+Diseases+-+Google+Search.png https://www.roepmanlab.com/therapies-for-renal-ciliopathies daily 0.75 2024-01-15 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/830bdd20-4ed8-44d3-a210-af1617813e02/European_Commission.svg.png https://www.roepmanlab.com/harnessing-autophagy-to-combat-macular-degeneration daily 0.75 2024-01-02 https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/1588184788383-ZIUKQTZP3XUS7PO7VV42/uitzicht+Logo1.jpg https://images.squarespace-cdn.com/content/v1/5e7bb7e41265894adacf1a3e/501d028b-8c60-4a04-ae43-11aea6f83924/Usher-UAD-23-10-1-scaled.jpg https://www.roepmanlab.com/basic-molecular-biology-techniques daily 0.75 2024-01-03 https://www.roepmanlab.com/make-a-reservation daily 0.75 2025-05-12